Industrial News
-
Primer design-detection specificity, stability and amplification efficiency
Specificity of detection In most cases, the purpose of primer design is to maximize the specificity of PCR. This is determined by the more or less predictable influence of many variables. One important variable is the sequence at the 3′end of the primer. Importantly, PCR assays designed for...Read more -
Nucleic acid drugs have entered the golden age, what are the key technologies that need to be solved urgently?
Source: Medical Micro After the COVID-19 outbreak, two mRNA vaccines were quickly approved for marketing, which has attracted more attention to the development of nucleic acid drugs. In recent years, a number of nucleic acid drugs that have the potential to become blockbuster drugs have published...Read more -
Analysis of Confirmation Indexes of Primer Probes in the Preliminary Period of PCR Reagents
Verifying the performance of primers and probes in the early stage of PCR reagents and determining the most suitable reaction conditions are the prerequisites to ensure the smooth progress of formal experiments. So how do we need to confirm the primer probe in the early ...Read more -
Sampling knowledge points that inspectors must see
Laboratory testing begins with sample collection, and sample collection is the easiest to overlook. The most important thing for sample collection is to select the correct sample type, use appropriate sampling tools, and carry out reasonable transportation and processing. I.Sample type Common sam...Read more -
The ultimate solution to nucleic acid aerosol pollution
PCR methods and nucleic acid aerosol contamination in nucleic acid testing laboratories are like two sides of a coin. We can only choose to have it or not, but we can’t choose whether we want it or spend it. 1. Screening of DNA remover To achieve the spatial remova...Read more -
Rapid identification of transgenic plants
As a new one in the laboratory, it is not a good job to screen out positive plants from a bunch of plants with a low conversion rate. Firstly, DNA must be extracted from a large number of samples one by one, and then the foreign genes will be detected by PCR. However, the results are often blanks...Read more -
A comprehensive interpretation of the key points of the SARS-CoV-2 nucleic acid test, why are there false negatives and retest positives?
In the early stage of the outbreak, due to the rapid development, rapid diagnosis of suspected patients is the key to preventing COVID-19. Some approved nucleic acid detection reagents have a short development time, and there are problems such as hurried performance confirmation, insufficient rea...Read more -
What is SNP?Topics on Population Genetics
The three letters SNP are ubiquitous in the study of population genetics. Regardless of human disease research, crop trait positioning, animal evolution and molecular ecology, SNPs are needed as the basis. However, if you don’t have a deep understanding of modern genetic...Read more -
How to improve the efficiency of LncRNA reverse transcription?
lncRNA features: 1. lncRNAs are usually longer, with dynamic expression and different splicing methods during differentiation; 2. Compared with coding genes, lncRNA is usually lower; 3. Most lncRNAs have obvious temporal and spatial expression specificity in the process ...Read more -
Four Main Solutions For PCR Product Pollution Control
1：Replace the experimental supplies in time Set up (NTC) negative control and repeat it many times. Once it is found that there is PCR product contamination in the laboratory, replace all experimental supplies in time. Such as: re-dilute and prepare the primers, re-sterilize the pipette tip, E...Read more -
Two dual-function RT-PCR enzymes
Traditional reverse transcriptases cannot tolerate high temperatures (the optimal temperature for MMLV activity is 37-50°C, and AMV is 42-60°C). The more complex viral RNA cannot be effectively reverse transcribed into cDNA at low temperatures, resulting in detection efficiency The reduction. Tra...Read more -
Nucleic acid isothermal amplification technology
PCR is the most widely used nucleic acid amplification technology and is widely used due to its sensitivity and specificity. However, PCR requires repeated thermal denaturation and cannot get rid of the limitations of relying on instruments and equipment, which limits its application in clinical ...Read more
