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nucleic acid testing and sampling room

Kit Description:

This kit uses Real-time RT PCR technology(rRT-PCR) for qualitative detection of SARS-CoV-2 (ORF1ab gene and N gene) nucleic acids in human nasopharyngeal or oropharyngeal swab samples, by multiplex PCR fluorescent probe method.This kit contains an internal control to assess specimen quality, thus no need for nucleic acid purification, can complete test in 1 hour, especially suitable for large-scale rapid detection.


Product Detail

Product Tags

Our target should be to consolidate and enhance the high quality and service of existing goods, meanwhile frequently develop new products and solutions to fulfill distinct customers’ demands for nucleic acid testing and sampling room, We promise to try our greatest to deliver you with premium quality and efficient solutions.
Our target should be to consolidate and enhance the high quality and service of existing goods, meanwhile frequently develop new products and solutions to fulfill distinct customers’ demands for nucleic acid testing and sampling room, With more than 9 years of experience and a professional team, now we have exported our solutions to many countries and regions all over the world. We welcome customers, business associations and friends from all parts of the world to contact us and seek cooperation for mutual benefits.


Specifications

48 Rxns, 96 Rxns

No.

Component

Amount

Main components

48 Rxns

96 Rxns

1 Nucleic acid release agent 1.4mL/tube 2 tubes 5.3 mL/bottle 1 bottle Surfactant
2 RNA protectant 27 μL/tube 1 tube 53 μL/tube 1 tube RNase inhibitor
3 SARS-CoV-2 reaction solution 800 μL/tube 1 tube 1600 μL/tube 1 tube Primer, probe, reaction buffer, dNTP
4 SARS-CoV-2 enzyme mixture 80 μL/tube 1 tube 160 μL/tube 1 tube Hot start Taq enzyme, M-MLV enzyme
5 SARS-CoV-2 positive control 100 μL/tube 1 tube 100 μL/tube 1 tube Recombinant plasmid containing target fragment, RNA
6 SARS-CoV-2 negative control 1200 μL/tube 1 tube 1200 μL/tube 1 tube TE buffer

Features&advantages

Quick and easy.
Avoid nucleic acid loss.
Low detection limit and high sensitivity.
Low equipment requirement and highly flexible use.

Detection sensitivity can reach the lowest level as 500 copies/mL).

GCE/mL Ct value
N gene ORF1ab gene
2.00E+08 16.43 15.16
2.00E+07 19.48 18.58
2.00E+06 22.71 21.86
2.00E+05 25.95 25.33
2.00E+04 29.30 28.49
2.00E+03 32.37 31.37
2.00E+02 35.32 34.47
2.00E+01 38.29 38.27
2.00E+00 N/A N/A
2.00E-01 N/A N/A
NC N/A N/A

Linear amplification curve

amplification curve-RT-qPCR

How to use

1. Applicable instrument

covid-19 nucleic acid detection kit1

2. Detection procedure

covid-19 nucleic acid detection kit2

Sample processing:Naopharyngeal or oropharyngeal swab sample (only 1 step operation)(Wait for 10 mins)

PCR amplificaton(<=55min)

Workflow:

covid-19 nucleic acid detection kit4

Storage

sealed from light and stored at -20±5℃;

storage
storage2

Shelf life: 1 year

Our target should be to consolidate and enhance the high quality and service of existing goods, meanwhile frequently develop new products and solutions to fulfill distinct customers’ demands for Factory supplied China FDA Sterile Medical Nylon Flock Flocked Sampling Specimen Collection Throat Nasopharyngeal Flocked Swabs Liquid Transport Medium, We promise to try our greatest to deliver you with premium quality and efficient solutions.
Factory supplied China Swab, Injection Instrument, With more than 9 years of experience and a professional team, now we have exported our solutions to many countries and regions all over the world. We welcome customers, business associations and friends from all parts of the world to contact us and seek cooperation for mutual benefits.


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