No matter new shopper or old customer, We believe in very long expression and dependable relationship for OEM Supply High Quality Magnetic Beads Virus DNA Nucleic Acid Extraction or Purification Kit, We warmly welcome clients, enterprise associations and pals from all around the earth to get in touch with us and seek out cooperation for mutual added benefits.
No matter new shopper or old customer, We believe in very long expression and dependable relationship for China PCR and Nucleic Acid Test Kit, Whether selecting a current product from our catalog or seeking engineering assistance for your application, you can talk to our customer service center about your sourcing requirements. We’ve been looking forward to cooperating with friends from all over the world.
Instruction Manuals:

 No matter new shopper or old customer, We believe in very long expression and dependable relationship for OEM Supply High Quality Magnetic Beads Virus DNA Nucleic Acid Extraction or Purification Kit, We warmly welcome clients, enterprise associations and pals from all around the earth to get in touch with us and seek out cooperation for mutual added benefits.
OEM Supply China PCR and Nucleic Acid Test Kit, Whether selecting a current product from our catalog or seeking engineering assistance for your application, you can talk to our customer service center about your sourcing requirements. We’ve been looking forward to cooperating with friends from all over the world.

Problem Analysis Guide

The following is an analysis of the problems that may be encountered in the extraction of viral DNA/RNA, hoping to be helpful to your experiments. In addition, for other experimental or technical problems other than operation instructions and problem analysis, we have dedicated technical support to help you. If you have any needs, please contact us: 028-83360257 or E-mail:

Tech@foregene.com.

No nucleic acid extraction or low nucleic acid yield

There are usually many factors that affect the recovery efficiency, such as: sample nucleic acid content, operation method, elution volume, etc.

Analysis of common causes:

1. An ice bath or low temperature (4°C) centrifugation was performed during the procedure.

Suggestion: Operate at room temperature (15-25°C) throughout the whole process, do not ice bath and low temperature centrifugation.

2. The sample was stored improperly or the sample was stored for too long.

Recommendation: Store samples at -80°C and avoid repeated freezing and thawing; try to use freshly collected samples for nucleic acid extraction.

3. Insufficient sample lysis.

Recommendation: Please ensure that the sample and lysis working solution are thoroughly mixed and incubated at room temperature (15-25°C) for 10 minutes.

4. Incorrect addition of eluent.

Suggestion: Make sure that RNase-Free ddH2O is added dropwise to the middle of the purification column membrane, and do not drop it on the purification column ring.

5. The correct volume of absolute ethanol was not added to Buffer RW2.

Suggestion: Please follow the instructions, add the correct volume of absolute ethanol to Buffer RW2 and mix well before using the kit.

6. Inappropriate sample volume.

Suggestion: 200µl of sample is processed for every 500µl of Buffer DRL. Excessive sample processing will result in lower nucleic acid extraction yield.

7. Inappropriate elution volume or incomplete elution.

Recommendation: The eluent volume of the purification column is 30-50μl; if the elution effect is not satisfactory, it is recommended to extend the time at room temperature after adding preheated RNase-Free ddH2O, such as 5-10min.

8. Ethanol remains on the column after washing with Buffer RW2.

Suggestion: If ethanol remains after centrifugation with Buffer RW2 for 2 minutes, the column can be placed at room temperature for 5 minutes after centrifugation to fully remove residual ethanol.

The purified nucleic acid is degraded

The quality of the purified nucleic acid is related to the preservation of the sample, RNase contamination, operation and other factors. Analysis of common causes:

1. The collected samples were not stored in time.

Suggestion: If the sample is not used in time after collection, please store it at -80°C at low temperature immediately. For RNA extraction, try to use freshly collected samples.

2. Collect samples and freeze and thaw repeatedly.

Suggestion: Avoid freezing and thawing (not more than once) during the collection and storage of samples, otherwise the nucleic acid yield will be reduced.

3. RNase is introduced in the operation room or disposable gloves, masks, etc. are not worn.

Recommendation: RNA extraction experiments are best performed in a separate RNA operation room, and the laboratory table should be cleaned before the experiment.

Wear disposable gloves and masks during the experiment to avoid RNA degradation caused by the introduction of RNase to the greatest extent.

4. The reagent was contaminated with RNase during use.

Recommendation: Replace with a new Viral DNA/RNA Isolation Kit for related experiments.

5. The centrifuge tubes and pipette tips used for RNA manipulation are contaminated with RNase.

Suggestion: Make sure that the centrifuge tubes, pipette tips, pipettes, etc. used for RNA extraction are all RNase-Free.

Purified nucleic acid affects downstream experiments

DNA and RNA purified by the purification column, if the salt ion and protein content are too high, it will affect the downstream experiments, such as: PCR amplification, reverse transcription, etc.

1. The eluted DNA and RNA have residual salt ions.

Suggestion: Make sure that the correct volume of absolute ethanol is added to Buffer RW2, and wash the purification column twice at the centrifugation speed specified in the operating instructions; Perform centrifugation to minimize salt ion contamination.

2. The eluted DNA and RNA have ethanol residues.

Suggestion: After confirming the washing with Buffer RW2, perform empty tube centrifugation at the centrifugation speed in the operating instructions; if there is still ethanol residue, you can centrifuge the empty tube and then place it at room temperature for 5 minutes to remove the ethanol residue to the greatest extent.            

Instruction Manuals:

Viral DNA&RNA Isolation Kit Instruction Manual