Specifications

50 Preps, 200 Preps

The kit uses the spin column and formula developed by Foregene, which can efficiently extract high-purity and high-quality total RNA from various plant tissues with low polysaccharides and polyphenols content. For plant samples with high polysaccharides or polyphenols content, it is recommended to use Plant Total RNA Isolation Plus Kit to get better RNA extraction results. The kit provides the DNA-Cleaning column that can easily remove genomic DNA from the supernatant and tissue lysate. RNA-only column can effectively bind RNA. The kit can process a large number of samples at the same time.

The entire system does not contain RNase, so the purified RNA will not be degraded. Buffer PRW1 and Buffer PRW2 can ensure that the RNA obtained is not contaminated by protein, DNA, ions, and organic compounds.

Kit components

Features&advantages

■ Operation at room temperature (15-25℃) throughout the whole process, without ice bath and low temperature centrifugation.
■ Complete kit RNase-Free, no need to worry about RNA degradation.
■ DNA-Cleaning Column specifically binds to DNA, so that the kit can remove genomic DNA contamination without adding DNase.
■ High RNA yield: RNA-only Column and unique formula can efficiently purify RNA.
■ Fast speed: easy to operate and can be completed within 30 minutes.
■ Safety: no organic reagent is required.
■ High quality: The purified RNA fragments are of high purity, free of protein and other impurities, and can meet various downstream experimental applications.

Kit application

It is suitable for the extraction and purification of total RNA from fresh or frozen plant tissue samples (especially fresh plant leaf tissue) with low polysaccharide and polyphenol content.

Work flow

Diagram

Plant Total RNA Isolation Kit Plus processed 50mg of fresh leaves of polysaccharides and polyphenols, and 5% purified RNA was tested by electrophoresis.
1: Banana
2: Ginkgo
3: Cotton
4: Pomegranate

Storage and shelf life

The kit can be stored for 12 months at room temperature (15–25 ℃) in dry environment, and 2–8 ℃ for longer time(24 months).

Buffer PSL1 can be stored at 4 ℃ for 1 month after adding 2-hydroxy-1-ethanethiol(optional).