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PCR Reagent

Kit Description:

The 2X Real PCR EasyTM  Mix-SYBR provided by the Real Time PCR EasyTM-SYBR Green I kit is a new premix system that uses SYBR Green I for Real Time PCR amplification reactions, which can greatly improve product specificity and reaction sensitivity . ROX is provided as internal control dye. The fluorescence intensity of this kit is 3-5 times that of similar products, which can be more sensitive and intuitively reflect the concentration of target template DNA.

2X Real PCR EasyTM  Mix-SYBR contains Foregene’s unique hot-start Taq DNA Polymerase. Compared with ordinary Taq enzymes, it has the advantages of high amplification efficiency, strong specific amplification ability and low mismatch rate. It can reduce non-specific amplification and improve the accuracy of PCR.


Product Detail

Product Tags

Our commission should be to serve our end users and purchasers with finest top quality and competitive portable digital products and solutions for PCR Reagent, We sincerely welcome the two overseas and domestic business enterprise associates, and hope to operate along with you in the around long term!
Our commission should be to serve our end users and purchasers with finest top quality and competitive portable digital products and solutions for PCR Reagent, With nearly 30 years’ experience in business, we’ve been confident in superior service, quality and delivery. We warmly welcome customers from all over the world to cooperate with our company for common development.

Specifications

5ml, 10ml, 40ml

Kit components

0.1U Taq Hero/μl

400μM dNTPs

20mM Tris-HCl(pH8.8)

100mM KCl

5mM MgCl2

Other stabilizers and enhancers
DNA Loading Buffer

Features&advantages

■  Higher fidelity: 6 times that of ordinary Taq enzyme;

■ Faster amplification speed : the amplification speed is 5-10sec/kb, which is 6-12 times that of ordinary Taq enzyme;

■ More template adaptability: It can efficiently amplify various complex DNA templates with high GC content;

■ Higher amplification efficiency: lower amplification cycles than ordinary Taq enzyme;

■ Environmental tolerance is stronger: placed at 37°C for a week, maintaining more than 90% activity;

■ It has 5′ →3′ DNA polymerase activity and 5′→3′ exonuclease activity, without 3′ →5′ exonuclease activity.

■ The kit is pre-mixed with Electrophoresis Loading Buffer, after PCR is completed, the sample can be directly loaded for electrophoresis; the operation is simple and easy.

Kit application

PCR amplification of DNA fragments, DNA labeling, DNA sequencing,PCR plus A tail

Storage and Shelf life

It can be stored for a long time at -20°C; for frequent use, it can be stored at 4°C for a short time.The 2×Real PCR EasyTM Mix-SYBR provided by the Real Time PCR EasyTM-SYBR Green I kit is a new premix system that uses SYBR Green I for Real Time PCR amplification reactions, which can greatly improve product specificity and reaction sensitivity . ROX is provided as internal control dye. The fluorescence intensity of this kit is 3-5 times that of similar products, which can be more sensitive and intuitively reflect the concentration of target template DNA.
2×Real PCR EasyTM Mix-SYBR contains Foregene’s unique hot-start Taq DNA Polymerase. Compared with ordinary Taq enzymes, it has the advantages of high amplification efficiency, strong specific amplification ability and low mismatch rate. It can reduce non-specific amplification and improve the accuracy of PCR.


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