PCR Reagent
Our commission should be to serve our end users and purchasers with finest top quality and competitive portable digital products and solutions for PCR Reagent, We sincerely welcome the two overseas and domestic business enterprise associates, and hope to operate along with you in the around long term!
Our commission should be to serve our end users and purchasers with finest top quality and competitive portable digital products and solutions for PCR Reagent, With nearly 30 years’ experience in business, we’ve been confident in superior service, quality and delivery. We warmly welcome customers from all over the world to cooperate with our company for common development.
Specifications
5ml, 10ml, 40ml
Kit components
0.1U Taq Hero/μl |
400μM dNTPs |
20mM Tris-HCl(pH8.8) |
100mM KCl |
5mM MgCl2 |
Other stabilizers and enhancers |
DNA Loading Buffer |
Features&advantages
■ Higher fidelity: 6 times that of ordinary Taq enzyme;
■ Faster amplification speed : the amplification speed is 5-10sec/kb, which is 6-12 times that of ordinary Taq enzyme;
■ More template adaptability: It can efficiently amplify various complex DNA templates with high GC content;
■ Higher amplification efficiency: lower amplification cycles than ordinary Taq enzyme;
■ Environmental tolerance is stronger: placed at 37°C for a week, maintaining more than 90% activity;
■ It has 5′ →3′ DNA polymerase activity and 5′→3′ exonuclease activity, without 3′ →5′ exonuclease activity.
■ The kit is pre-mixed with Electrophoresis Loading Buffer, after PCR is completed, the sample can be directly loaded for electrophoresis; the operation is simple and easy.
Kit application
PCR amplification of DNA fragments, DNA labeling, DNA sequencing,PCR plus A tail
Storage and Shelf life
It can be stored for a long time at -20°C; for frequent use, it can be stored at 4°C for a short time.The 2×Real PCR EasyTM Mix-SYBR provided by the Real Time PCR EasyTM-SYBR Green I kit is a new premix system that uses SYBR Green I for Real Time PCR amplification reactions, which can greatly improve product specificity and reaction sensitivity . ROX is provided as internal control dye. The fluorescence intensity of this kit is 3-5 times that of similar products, which can be more sensitive and intuitively reflect the concentration of target template DNA.
2×Real PCR EasyTM Mix-SYBR contains Foregene’s unique hot-start Taq DNA Polymerase. Compared with ordinary Taq enzymes, it has the advantages of high amplification efficiency, strong specific amplification ability and low mismatch rate. It can reduce non-specific amplification and improve the accuracy of PCR.