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Short Lead Time for China Thermal Cycler PCR Instrument Machine

Kit Description:

This kit uses Real-time RT PCR technology(rRT-PCR) for qualitative detection of SARS-CoV-2 (ORF1ab gene and N gene) nucleic acids in human nasopharyngeal or oropharyngeal swab samples, by multiplex PCR fluorescent probe method.This kit contains an internal control to assess specimen quality, thus no need for nucleic acid purification, can complete test in 1 hour, especially suitable for large-scale rapid detection.


Product Detail

Product Tags

We have now our possess revenue group, design staff, technical crew, QC team and package group. We now have strict excellent regulate procedures for each process. Also, all of our workers are experienced in printing subject for Short Lead Time for China Thermal Cycler PCR Instrument Machine, Now we have four leading solutions. Our merchandise are greatest sold not only in the Chinese sector, but also welcomed from the international market.
We have now our possess revenue group, design staff, technical crew, QC team and package group. We now have strict excellent regulate procedures for each process. Also, all of our workers are experienced in printing subject for China PCR Instrument, Thermal Cycler, We insist on “Quality First, Reputation First and Customer First”. We are committed to providing high-quality items and good after-sales services. Up to now, our solutions have been exported to more than 60 countries and areas around the world, such as America, Australia and Europe. We enjoy a high reputation at home and abroad. Always persisting in the principle of “Credit, Customer and Quality”, we expect cooperation with people in all walks of life for mutual benefits.


Specifications

48 Rxns, 96 Rxns

No.

Component

Amount

Main components

48 Rxns

96 Rxns

1 Nucleic acid release agent 1.4mL/tube 2 tubes 5.3 mL/bottle 1 bottle Surfactant
2 RNA protectant 27 μL/tube 1 tube 53 μL/tube 1 tube RNase inhibitor
3 SARS-CoV-2 reaction solution 800 μL/tube 1 tube 1600 μL/tube 1 tube Primer, probe, reaction buffer, dNTP
4 SARS-CoV-2 enzyme mixture 80 μL/tube 1 tube 160 μL/tube 1 tube Hot start Taq enzyme, M-MLV enzyme
5 SARS-CoV-2 positive control 100 μL/tube 1 tube 100 μL/tube 1 tube Recombinant plasmid containing target fragment, RNA
6 SARS-CoV-2 negative control 1200 μL/tube 1 tube 1200 μL/tube 1 tube TE buffer

Features&advantages

Quick and easy.
Avoid nucleic acid loss.
Low detection limit and high sensitivity.
Low equipment requirement and highly flexible use.

Detection sensitivity can reach the lowest level as 500 copies/mL).

GCE/mL Ct value
N gene ORF1ab gene
2.00E+08 16.43 15.16
2.00E+07 19.48 18.58
2.00E+06 22.71 21.86
2.00E+05 25.95 25.33
2.00E+04 29.30 28.49
2.00E+03 32.37 31.37
2.00E+02 35.32 34.47
2.00E+01 38.29 38.27
2.00E+00 N/A N/A
2.00E-01 N/A N/A
NC N/A N/A

Linear amplification curve

amplification curve-RT-qPCR

How to use

1. Applicable instrument

covid-19 nucleic acid detection kit1

2. Detection procedure

covid-19 nucleic acid detection kit2

Sample processing:Naopharyngeal or oropharyngeal swab sample (only 1 step operation)(Wait for 10 mins)

PCR amplificaton(<=55min)

Workflow:

covid-19 nucleic acid detection kit4

Storage

sealed from light and stored at -20±5℃;

storage
storage2

Shelf life: 1 year

We have now our possess revenue group, design staff, technical crew, QC team and package group. We now have strict excellent regulate procedures for each process. Also, all of our workers are experienced in printing subject for Short Lead Time for China Thermal Cycler PCR Instrument Machine, Now we have four leading solutions. Our merchandise are greatest sold not only in the Chinese sector, but also welcomed from the international market.
Short Lead Time for China PCR Instrument, Thermal Cycler, We insist on “Quality First, Reputation First and Customer First”. We are committed to providing high-quality items and good after-sales services. Up to now, our solutions have been exported to more than 60 countries and areas around the world, such as America, Australia and Europe. We enjoy a high reputation at home and abroad. Always persisting in the principle of “Credit, Customer and Quality”, we expect cooperation with people in all walks of life for mutual benefits.


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