We’ve been experienced manufacturer. Wining the majority of your crucial certifications of its market for Super Purchasing for China Real-Time PCR, Should you be interested in almost any goods, remember to truly feel totally free to get in touch with us for further facts or be sure to deliver us email right, we will reply you in just 24 hrs as well as the finest quotation are going to be provided.
We’ve been experienced manufacturer. Wining the majority of your crucial certifications of its market for China PCR, Real Time Pcr, With the enterprising spirit of” high efficiency, convenience, practicality and innovation”, and in line with such serving guidance of “good quality but better price, ” and “global credit”, we are striving to cooperate with the automobile parts companies all over the world to make a win-win partnership.

Descriptions

This kit uses a unique lysis buffer system that can quickly release RNA from cultured cell samples for RT-qPCR reactions, thereby eliminating the time-consuming and laborious RNA purification process. The RNA template can be obtained in just 7 minutes. The 5×Direct RT Mix and 2×Direct qPCR Mix-SYBR reagents provided by the kit can quickly and effectively obtain real-time quantitative PCR results.

5×Direct RT Mix and 2×Direct qPCR Mix-SYBR have strong inhibitor tolerance, and the lysate of the samples can be used as the template for RT-qPCR directly. This kit contains the unique RNA high-affinity Foregene reverse transcriptase, and Hot D-Taq DNA polymerase, dNTPs, MgCl₂, reaction buffer, PCR optimizer and stabilizer.

Specifications

200×20μl Rxns, 1000×20μl Rxns

Kit components

Features&advantages

■  Simple and effective : with Cell Direct RT technology, RNA samples can be obtained in just 7 minutes.

■ The sample demand is small, as low as 10 cells can be tested.

■ High throughput: it can quickly detect RNA in cells cultured in 384, 96, 24, 12, 6-well plates.

■ DNA Eraser can quickly remove released genomes, greatly reduce the impact on subsequent experimental results.

■ Optimized RT and qPCR system makes the two-step RT-PCR reverse transcription more efficient and PCR more specific, and more resistant to RT-qPCR reaction inhibitors.

Kit application

Scope of application: cultured cells.

- RNA released by sample lysis: only applicable to the RT-qPCR template of this kit.

- The kit can be used for the following purposes: gene expression analysis, verification of siRNA-mediated gene silencing effect, drug screening, etc.

Diagram

Storage and Shelf life

Part I of this kit should be stored at 4℃; Part II should be stored at -20℃.

Foregene Protease Plus II should be stored at 4℃,do not freeze at -20℃.

Reagent 2×Direct qPCR Mix-SYBR should be stored at -20℃ in the dark; if used frequently, it can also be stored at 4℃ for short-term storage (use up within 10 days).We’ve been experienced manufacturer. Wining the majority of your crucial certifications of its market for Super Purchasing for China Q160/Q320 Real-Time PCR, Should you be interested in almost any goods, remember to truly feel totally free to get in touch with us for further facts or be sure to deliver us email right, we will reply you in just 24 hrs as well as the finest quotation are going to be provided.
Super Purchasing for China PCR, Real Time PCR, With the enterprising spirit of” high efficiency, convenience, practicality and innovation”, and in line with such serving guidance of “good quality but better price, ” and “global credit”, we are striving to cooperate with the automobile parts companies all over the world to make a win-win partnership.