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Top Grade China Nucleic Acid Extraction System, Fully Automatic Nucleic Acid Purification Extraction Machine, 32 & 96 Type CE

Kit Description:

This kit uses a unique lysis buffer system that can quickly release RNA from cultured cell samples for RT-qPCR reactions, thereby eliminating the time-consuming and laborious RNA purification process. The RNA template can be obtained in just 7 minutes. The 5×Direct RT Mix and 2×Direct qPCR Mix-SYBR reagents provided by the kit can quickly and effectively obtain real-time quantitative PCR results.

5×Direct RT Mix and 2×Direct qPCR Mix-SYBR have strong inhibitor tolerance, and the lysate of the samples can be used as the template for RT-qPCR directly. This kit contains the unique RNA high-affinity Foregene reverse transcriptase, and Hot D-Taq DNA polymerase, dNTPs, MgCl2, reaction buffer, PCR optimizer and stabilizer.


Product Detail

Product Tags

Our mission is usually to turn into an innovative provider of high-tech digital and communication devices by furnishing benefit added design and style, world-class manufacturing, and service capabilities for Top Grade China Nucleic Acid Extraction System, Fully Automatic Nucleic Acid Purification Extraction Machine, 32 & 96 Type CE, High-quality is factory’s everyday living , Focus on customers’ demand would be the source of organization survival and advancement, We adhere to honesty and good faith doing the job attitude, seeking forward towards your coming !
Our mission is usually to turn into an innovative provider of high-tech digital and communication devices by furnishing benefit added design and style, world-class manufacturing, and service capabilities for Automatic Nucleic Acid Purification, China Nucleic Acid, we are now looking forward to even greater cooperation with overseas customers based on mutual benefits. We will work wholeheartedly to improve our products and services. We also promise to work jointly with business partners to elevate our cooperation to a higher level and share success together. Warmly welcome you to visit our factory sincerely.

Specifications

200×20μl Rxns, 1000×20μl Rxns

Kit components

Part I

Buffer CL

Foregene Protease Plus II

Buffer ST

Part II

DNA Eraser

5× Direct RT Mix

2× Direct qPCR Mix-SYBR

50× ROX Reference Dye

RNase-Free ddH2O

Instructions

Features&advantages

■  Simple and effective : with Cell Direct RT technology, RNA samples can be obtained in just 7 minutes.

■ The sample demand is small, as low as 10 cells can be tested.

■ High throughput: it can quickly detect RNA in cells cultured in 384, 96, 24, 12, 6-well plates.

■ DNA Eraser can quickly remove released genomes, greatly reduce the impact on subsequent experimental results.

■ Optimized RT and qPCR system makes the two-step RT-PCR reverse transcription more efficient and PCR more specific, and more resistant to RT-qPCR reaction inhibitors.

Kit application

Scope of application: cultured cells.

- RNA released by sample lysis: only applicable to the RT-qPCR template of this kit.

- The kit can be used for the following purposes: gene expression analysis, verification of siRNA-mediated gene silencing effect, drug screening, etc.

Diagram

Cell Direct RT qPCR diagram

Storage and Shelf life

Part I of this kit should be stored at 4℃; Part II should be stored at -20℃.

Foregene Protease Plus II should be stored at 4℃,do not freeze at -20℃.

Reagent 2×Direct qPCR Mix-SYBR should be stored at -20℃ in the dark; if used frequently, it can also be stored at 4℃ for short-term storage (use up within 10 days).Our mission is usually to turn into an innovative provider of high-tech digital and communication devices by furnishing benefit added design and style, world-class manufacturing, and service capabilities for Top Grade China Nucleic Acid Extraction System, Fully Automatic Nucleic Acid Purification Extraction Machine, 32 & 96 Type CE, High-quality is factory’s everyday living , Focus on customers’ demand would be the source of organization survival and advancement, We adhere to honesty and good faith doing the job attitude, seeking forward towards your coming !
Top Grade China Nucleic Acid, Automatic Nucleic Acid Purification, we are now looking forward to even greater cooperation with overseas customers based on mutual benefits. We will work wholeheartedly to improve our products and services. We also promise to work jointly with business partners to elevate our cooperation to a higher level and share success together. Warmly welcome you to visit our factory sincerely.


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