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Wholesale ODM China Lab Using Peltier-Based Thermal Cycler PCR

Kit Description:

This kit uses Real-time RT PCR technology(rRT-PCR) for qualitative detection of SARS-CoV-2 (ORF1ab gene and N gene) nucleic acids in human nasopharyngeal or oropharyngeal swab samples, by multiplex PCR fluorescent probe method.This kit contains an internal control to assess specimen quality, thus no need for nucleic acid purification, can complete test in 1 hour, especially suitable for large-scale rapid detection.

20+ years R&D Manufacturing Experience         25+ highest impact factor                       

1000+ different lab reagents                                100+ universities partner                         

400+ distributors                                                   2000+ SCI citations of the lab reagents 


Product Detail

Product Tags

The organization keeps for the procedure concept “scientific administration, superior quality and effectiveness primacy, shopper supreme for Wholesale ODM China Lab Using Peltier-Based Thermal Cycler PCR, If needed, welcome to make contact with us by our web page or phone consultation, we will be delighted to serve you.
The organization keeps for the procedure concept “scientific administration, superior quality and effectiveness primacy, shopper supreme for China PCR, Thermal Cycler, Due to the changing trends in this field, we involve ourselves into goods trade with dedicated efforts and managerial excellence. We maintain timely delivery schedules, innovative designs, quality and transparency for our customers. Our moto is to deliver quality solutions within stipulated time.

Specifications

48 Rxns, 96 Rxns

No.

Component

Amount

Main components

48 Rxns

96 Rxns

1 Nucleic acid release agent 1.4mL/tube 2 tubes 5.3 mL/bottle 1 bottle Surfactant
2 RNA protectant 27 μL/tube 1 tube 53 μL/tube 1 tube RNase inhibitor
3 SARS-CoV-2 reaction solution 800 μL/tube 1 tube 1600 μL/tube 1 tube Primer, probe, reaction buffer, dNTP
4 SARS-CoV-2 enzyme mixture 80 μL/tube 1 tube 160 μL/tube 1 tube Hot start Taq enzyme, M-MLV enzyme
5 SARS-CoV-2 positive control 100 μL/tube 1 tube 100 μL/tube 1 tube Recombinant plasmid containing target fragment, RNA
6 SARS-CoV-2 negative control 1200 μL/tube 1 tube 1200 μL/tube 1 tube TE buffer

Features&advantages

Quick and easy.
Avoid nucleic acid loss.
Low detection limit and high sensitivity.
Low equipment requirement and highly flexible use.

Detection sensitivity can reach the lowest level as 500 copies/mL).

GCE/mL Ct value
N gene ORF1ab gene
2.00E+08 16.43 15.16
2.00E+07 19.48 18.58
2.00E+06 22.71 21.86
2.00E+05 25.95 25.33
2.00E+04 29.30 28.49
2.00E+03 32.37 31.37
2.00E+02 35.32 34.47
2.00E+01 38.29 38.27
2.00E+00 N/A N/A
2.00E-01 N/A N/A
NC N/A N/A

Linear amplification curve

amplification curve-RT-qPCR

How to use

1. Applicable instrument

covid-19 nucleic acid detection kit1

2. Detection procedure

covid-19 nucleic acid detection kit2

Sample processing:Naopharyngeal or oropharyngeal swab sample (only 1 step operation)(Wait for 10 mins)

PCR amplificaton(<=55min)

Workflow:

covid-19 nucleic acid detection kit4

Storage

sealed from light and stored at -20±5℃;

storage
storage2

Shelf life: 1 yearThe organization keeps for the procedure concept “scientific administration, superior quality and effectiveness primacy, shopper supreme for Wholesale ODM China Lab Using Peltier-Based Thermal Cycler PCR, If needed, welcome to make contact with us by our web page or phone consultation, we will be delighted to serve you.
Wholesale ODM China PCR, Thermal Cycler, Due to the changing trends in this field, we involve ourselves into goods trade with dedicated efforts and managerial excellence. We maintain timely delivery schedules, innovative designs, quality and transparency for our customers. Our moto is to deliver quality solutions within stipulated time.


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