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Wholesale OEM China Laboratory Nucleic Acid Extractor Extraction Reagent Kit for PCR Detection

Kit Description:

This kit uses Real-time RT PCR technology(rRT-PCR) for qualitative detection of SARS-CoV-2 (ORF1ab gene and N gene) nucleic acids in human nasopharyngeal or oropharyngeal swab samples, by multiplex PCR fluorescent probe method.This kit contains an internal control to assess specimen quality, thus no need for nucleic acid purification, can complete test in 1 hour, especially suitable for large-scale rapid detection.


Product Detail

Product Tags

Excellent comes 1st; service is foremost; small business is cooperation” is our organization philosophy which is regularly observed and pursued by our company for Wholesale OEM China Laboratory Nucleic Acid Extractor Extraction Reagent Kit for PCR Detection, Sincerely hope we are growing up together with our customers all over the world.
Excellent comes 1st; service is foremost; small business is cooperation” is our organization philosophy which is regularly observed and pursued by our company for China Nucleic Acid Extractor, Nucleic Acid Extraction, Strong infrastructure is the need to have of any organization. We are backed with a robust infrastructural facility that enables us to manufacture, store, quality check and dispatch our solutions worldwide. To maintain smooth work flow, now we have sectioned our infrastructure into a number of departments. All these departments are functional with latest tools, modernized machines and equipment. Owing to which, we’re able to accomplish voluminous production without compromising on the quality.


Specifications

48 Rxns, 96 Rxns

No.

Component

Amount

Main components

48 Rxns

96 Rxns

1 Nucleic acid release agent 1.4mL/tube 2 tubes 5.3 mL/bottle 1 bottle Surfactant
2 RNA protectant 27 μL/tube 1 tube 53 μL/tube 1 tube RNase inhibitor
3 SARS-CoV-2 reaction solution 800 μL/tube 1 tube 1600 μL/tube 1 tube Primer, probe, reaction buffer, dNTP
4 SARS-CoV-2 enzyme mixture 80 μL/tube 1 tube 160 μL/tube 1 tube Hot start Taq enzyme, M-MLV enzyme
5 SARS-CoV-2 positive control 100 μL/tube 1 tube 100 μL/tube 1 tube Recombinant plasmid containing target fragment, RNA
6 SARS-CoV-2 negative control 1200 μL/tube 1 tube 1200 μL/tube 1 tube TE buffer

Features&advantages

Quick and easy.
Avoid nucleic acid loss.
Low detection limit and high sensitivity.
Low equipment requirement and highly flexible use.

Detection sensitivity can reach the lowest level as 500 copies/mL).

GCE/mL Ct value
N gene ORF1ab gene
2.00E+08 16.43 15.16
2.00E+07 19.48 18.58
2.00E+06 22.71 21.86
2.00E+05 25.95 25.33
2.00E+04 29.30 28.49
2.00E+03 32.37 31.37
2.00E+02 35.32 34.47
2.00E+01 38.29 38.27
2.00E+00 N/A N/A
2.00E-01 N/A N/A
NC N/A N/A

Linear amplification curve

amplification curve-RT-qPCR

How to use

1. Applicable instrument

covid-19 nucleic acid detection kit1

2. Detection procedure

covid-19 nucleic acid detection kit2

Sample processing:Naopharyngeal or oropharyngeal swab sample (only 1 step operation)(Wait for 10 mins)

PCR amplificaton(<=55min)

Workflow:

covid-19 nucleic acid detection kit4

Storage

sealed from light and stored at -20±5℃;

storage
storage2

Shelf life: 1 year

Excellent comes 1st; service is foremost; small business is cooperation” is our organization philosophy which is regularly observed and pursued by our company for Wholesale OEM China Laboratory Nucleic Acid Extractor Extraction Reagent Kit for PCR Detection, Sincerely hope we are growing up together with our customers all over the world.
Wholesale OEM China Nucleic Acid Extractor, Nucleic Acid Extraction, Strong infrastructure is the need to have of any organization. We are backed with a robust infrastructural facility that enables us to manufacture, store, quality check and dispatch our solutions worldwide. To maintain smooth work flow, now we have sectioned our infrastructure into a number of departments. All these departments are functional with latest tools, modernized machines and equipment. Owing to which, we’re able to accomplish voluminous production without compromising on the quality.


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