Specifications

50 Preps

Buffer FL1: Provides an environment for enzymatic hydrolysis of tissues.

Foregene Protease Plus: Enzymatically digest tissue samples in the environment of Buffer FL1.

Buffer FL2: Inactivate Foregene Protease Plus and provide a DNA loading environment.

Buffer PW: Remove impurities such as protein and RNA in DNA.

Buffer WB: Remove residual salt ions in DNA.

Buffer EB: Elute the DNA on the purification column membrane.

DNA-only Column: Specifically adsorb the genomic DNA in the lysate.

Kit components

Features&advantages

-No RNase contamination: The DNA-Only Column provided by the kit makes it possible to remove RNA from genomic DNA without adding RNase during the experiment, avoiding the laboratory from being contaminated by exogenous RNase.

-Fast speed: Foregene Protease Plus has higher activity than similar proteases, and it digests tissue samples faster; the operation is simple, and the genomic DNA extraction operation can be completed within 2-5 hours.

-Convenience: The centrifugation is performed at room temperature, and there is no need for 4°C low-temperature centrifugation or ethanol precipitation of DNA.

-Safety: no organic reagent extraction is required.

-High quality: The extracted genomic DNA fragments are large, no RNA, no RNase, and extremely low ion content, which can meet the requirements of various experiments.

Kit application

It is suitable for extraction and purification of genomic DNA from formaldehyde-fixed samples and paraffin-embedded tissue samples.

Workflow

Storage and Shelf life

-This kit can be stored for 12 months under dry conditions at room temperature (15-25°C); if it needs to be stored for a longer period of time, it can be stored at 2-8°C.

Note: If stored at low temperature, the solution is prone to precipitation. Before use, be sure to put the solution in the kit at room temperature for a period of time. If necessary, preheat it in a 37°C water bath for 10 minutes to dissolve the precipitate, and mix it before use.

-Foregene Protease Plus solution has a unique formula that is active when stored at room temperature for a long time (3 months); it is more active and stable when stored at 4°C, so it is recommended to store it at 4°C, remember not to keep it at -20°C.