General Plasmid Mini Kit General Plasmid DNA Extraction Miniprep Mini Kits
Kit Description:
Quickly purify high-quality plasmid DNA from transformed bacteria for routine molecular biology experiments such as transformation and enzyme digestion.
◮ Remove RNA without adding RNase
◮ Convenient—the centrifugation is performed at room temperature
◮ Fast—the operation can be completed in 20 minutes
◮ Safe—no organic reagent used
◮ High purity—OD260/280≈1.7-1.9
Kit Descriptions
The FOREGENE’S nucleic acid extraction and purification series products can be extracted and purified to high-quality nucleic acids from a variety of sources and complex component samples. The DNA extraction series products are based on DNA-Only silica membrane purification columns and unique formula reagents that can purify high-quality DNA from a variety of samples.
The General Plasmid Mini Kit uses DNA-Only purification column technology and an efficient SDS lysis formula to obtain high-quality plasmid DNA from bacteria within 20 minutes. The DNA-Only silica gel membrane used in the spin column is a unique new material of FOREGENE, which can adsorb DNA efficiently and specifically,and remove RNA, impurity proteins, ions and other organic compounds in cells to the greatest extent. The maximum binding capacity of plasmid DNA of DNA-Only purification column is 60μg DNA. The amount of plasmid DNA actually extracted is related to the volume of the bacterial solution, the number of plasmid copies (high copy, low copy), strains, culture conditions and other factors.
Specifications
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General Plasmid Mini Kit |
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Kit content |
DE-01001 |
DE-01002 |
DE-01003 |
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50T |
100T |
250T |
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Buffer S1 |
10ml |
20ml |
50ml |
|
Buffer S2 |
10ml |
20ml |
50ml |
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Buffer S3 |
25ml |
50ml |
125ml |
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Buffer PW |
25ml |
50ml |
125ml |
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Buffer WB2 |
15ml |
30ml |
75ml |
|
Buffer EB |
10ml |
20ml |
50ml |
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DNA-Only Column |
50 |
100 |
250 |
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Manual |
1 |
1 |
1 |
Features&advantages
- No RNase contamination: The DNA-Only Column provided by the kit makes it possible to remove the RNA in the plasmid DNA without adding RNase during the experiment, avoiding the contamination of the laboratory by exogenous RNase.
- Fast speed: Plasmid DNA extraction can be completed within 20 minutes.
- Safe: No organic reagent extraction is required.
- High quality: The plasmid obtained by extraction has no RNA, no RNase and extremely low ion content, which can meet the requirements of various experiments.
Kit application
Plasmid DNA extraction and purification for Gram-negative bacteria.
Work flow
Diagram
Storage and shelf life
This kit can be stored for 12 months under dry conditions at room temperature (15-25℃); if it needs to be stored for a longer time, it can be stored in 2–8℃.
Note: If stored at low temperature, the solution is prone to precipitation. Before use, be sure to place the solution in the kit at room temperature for a period of time. If necessary, preheat it in a 37℃ water bath for 10 minutes to dissolve the precipitate, and mix it before use.
