To fulfill the customers’ over-expected fulfillment , we have now our solid staff to deliver our greatest general assistance which includes internet marketing, product sales, creating, manufacturing, excellent controlling, packing, warehousing and logistics for High Performance China Factory OEM Thermal Cycler PCR Detection System Lab Quantitative Real Time PCR Machine for Hospital, Our goal is always to assist customers understand their ambitions. We’re earning terrific efforts to achieve this win-win circumstance and sincerely welcome you to definitely sign up for us!
To fulfill the customers’ over-expected fulfillment , we have now our solid staff to deliver our greatest general assistance which includes internet marketing, product sales, creating, manufacturing, excellent controlling, packing, warehousing and logistics for Automatic Nucleic Acid Detection, China Rt PCR Detection Instruments, Our factory insists on the principle of “Quality First, Sustainable Development”, and takes “Honest Business, Mutual Benefits” as our developable goal. All members sincerely thanks for all old and new customers’ support. We will keep working hard and offering you the highest-quality solutions and service.Thanks.

Kits Descriptions

50 Preps, 200 Preps

This kit uses the spin column and formula developed by our company, which can extract high-purity and high-quality total RNA from various animal tissues with high efficiency.It provides an efficient DNA-Cleaning Column, which can easily separate and adsorb genomic DNA from the supernatant and tissue lysate, simple and time-saving; RNA-only Column can efficiently bind RNA and can be processed simultaneously with a unique formula Lots of samples.

The whole system is RNase-Free, so that the extracted RNA is not degraded; Buffer RW1, Buffer RW2 buffer washing system, so that the obtained RNA is free of protein, DNA, ion, and organic compound pollution.

Kit components:

Features&advantages

■ No need to worry about RNA degradation; the whole system is RNase-Free
■ Effectively remove DNA-using DNA-Cleaning Column
■ Remove DNA without adding DNase
■ Simple-all operations are completed at room temperature
■ Fast -operation can be completed in 30 minutes
■ Safe-no organic reagent required
■ High purity -OD260/280≈1.8-2.1

Kit application

It is suitable for the extraction and purification of total RNA from a variety of fresh or frozen animal tissues or cultured cells.

Product parameters

■ Downstream applications: first-strand cDNA synthesis, RT-PCR, molecular cloning, Northern Blot, etc.
■ Samples: animal tissues, cultured cells
■ Dosage: Tissues 10-20mg, Cells(2-5)×10⁶
■ Maximum DNA binding capacity of purification column: 80 μg
■ Elution volume: 50-200 μl

Work flow

Diagram

Animal Total RNA Isolation Kit treated 20mg
Fresh mouse samples, take 5% purified total RNA 1% agar

Glycogel electrophoresis
1: Spleen 2: Kidney
3: Liver 4: Heart

Storage and shelf life

The kit can be stored for 24 months at room temperature (15–25 ℃) or 2–8 ℃ for longer time. Buffer RL1 can be stored at 4 ℃ for 1 month after adding β- mercaptoethanol (optional).

To fulfill the customers’ over-expected fulfillment , we have now our solid staff to deliver our greatest general assistance which includes internet marketing, product sales, creating, manufacturing, excellent controlling, packing, warehousing and logistics for High Performance China Factory OEM Thermal Cycler PCR Detection System Lab Quantitative Real Time PCR Machine for Hospital, Our goal is always to assist customers understand their ambitions. We’re earning terrific efforts to achieve this win-win circumstance and sincerely welcome you to definitely sign up for us!
High Performance China Rt PCR Detection Instruments, Automatic Nucleic Acid Detection, Our factory insists on the principle of “Quality First, Sustainable Development”, and takes “Honest Business, Mutual Benefits” as our developable goal. All members sincerely thanks for all old and new customers’ support. We will keep working hard and offering you the highest-quality solutions and service.Thanks.