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What is SNP?Topics on Population Genetics
The three letters SNP are ubiquitous in the study of population genetics. Regardless of human disease research, crop trait positioning, animal evolution and molecular ecology, SNPs are needed as the basis. However, if you don’t have a deep understanding of modern genetic...Read more -
How to improve the efficiency of LncRNA reverse transcription?
lncRNA features: 1. lncRNAs are usually longer, with dynamic expression and different splicing methods during differentiation; 2. Compared with coding genes, lncRNA is usually lower; 3. Most lncRNAs have obvious temporal and spatial expression specificity in the process ...Read more -
Dry goods | Nucleic acid extraction has bugs, it’s time to upgrade equipment!
Nucleic acid extraction is an introductory skill for molecular biology experiments. Whether it can master and use this skill proficiently has also become a prerequisite for later experimenters to upgrade to experimental masters. Ac...Read more -
Four Main Solutions For PCR Product Pollution Control
1：Replace the experimental supplies in time Set up (NTC) negative control and repeat it many times. Once it is found that there is PCR product contamination in the laboratory, replace all experimental supplies in time. Such as: re-dilute and prepare the primers, re-sterilize the pipette tip, E...Read more -
Two dual-function RT-PCR enzymes
Traditional reverse transcriptases cannot tolerate high temperatures (the optimal temperature for MMLV activity is 37-50°C, and AMV is 42-60°C). The more complex viral RNA cannot be effectively reverse transcribed into cDNA at low temperatures, resulting in detection efficiency The reduction. Tra...Read more -
Nucleic acid isothermal amplification technology
PCR is the most widely used nucleic acid amplification technology and is widely used due to its sensitivity and specificity. However, PCR requires repeated thermal denaturation and cannot get rid of the limitations of relying on instruments and equipment, which limits its application in clinical ...Read more -
What is an mRNA vaccine?
What is an mRNA vaccine The mRNA vaccine transfers RNA to the cells of the body to express and produce protein antigens after relevant modifications in vitro, thereby leading the body to produce an immune response against the antigen, thereby expanding the bod...Read more -
PCR Technology
PCR (polymerase chain reaction) is one of the in-vitro DNA amplification technologies, with a history of more than 30 years. PCR technology was pioneered by Kary Mullis of Cetus, USA in 1983. Mullis applied for a PCR patent in 1985 and published the first PCR academic paper on Science in the same...Read more -
New coronavirus detection, from nucleic acid, antibody to antigen, how to choose the normalization of the epidemic?
An epidemic has changed the world. Throughout the world, governments of all countries are facing huge challenges in epidemic prevention and control. During the COVID-19 pandemic, China is in the four stages of the prevention and response framework (prevention, detection, control and The key to su...Read more -
Four ways for preventing PCR products contamination
Building SOP Systerm Establish PCR experiment SOP to standardize the behavior of experiment personnel. The experimenters strictly abide by the operating procedures, and minimize the PCR pollution that may be caused by human factors or prevent the occurrence of pollution in the operation. In addi...Read more -
How to improve the efficiency of LncRNA reverse transcription?
Lead Long non-coding RNA, lncRNA is a non-coding RNA with a length greater than 200 nucleotides, generally between 200-100000 nt. lncRNA regulates gene expression at epigenetic, transcription and post-transcriptional levels, and participates in X chromosome silencing, genome imprinting and chroma...Read more -
It can be passed from person to person in 14 seconds. What happened to the “extremely contagious” Delta strain in Zhong Nanshan Pass?
Tranaslated source: WuXi AppTec team editor In Guangzhou, China, the police responsible for assisting the epidemiological investigation released a surveillance video: In the same restaurant, the two walked into the bathroom one after the other without any physical contact. Only 14 seconds of co-e...Read more
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