Mouse Tail Direct PCR Kit-UNG

Kit Description:

No time-consuming and expensive DNA purification is required.

The sample demand is small, as little as 5mg mouse tissue or 2-5mm mouse tail can be tested.

No special treatments such as grinding and crushing are required, and the operation is simple.

The optimized PCR system makes PCR have higher specificity and stronger tolerance to PCR reaction inhibitors.

Anti-pollution PCR system 2×M1-PCR EasyTM Mix (UNG), which effectively eliminates the pollution caused by PCR products and ensures the specificity and accuracy of amplification.

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Product Detail

Product Tags

Descriptions

This kit uses a unique lysis buffer system to quickly release genomic DNA from mouse tails, ears, muscles and other tissue samples for PCR reactions, so it is particularly suitable for large-scale genetic testing.

The process of releasing genomic DNA from the lysis buffer is completed within 10-30 minutes at 65°C. No other processes such as protein and RNA removal are required, and the released trace DNA can be used as a template for PCR reaction.

2× M1-PCR EasyTM Mix (UNG) uses dUTP instead of dTTP on the basis of 2× M1-PCR EasyTM Mix (UNG), and adds UNG enzyme (Uracil-N-glycosylase) that can degrade the template containing dUTP at the same time. Before the PCR reaction, the UNG enzyme is used to degrade the PCR product containing uracil. The UNG enzyme will not have any effect on the template that does not contain uracil, thereby ensuring the specificity and accuracy of amplification and preventing the possibility of large-scale genetic testing. Contamination of PCR products occurred.

D-Taq DNA polymerase is a DNA polymerase specially developed by Foregene for direct PCR reactions. D-Taq DNA polymerase has strong tolerance to a variety of PCR reaction inhibitors, and can efficiently amplify trace amounts of DNA in various complex reaction systems, and the amplification speed can reach 2Kb/min, which is especially suitable for Direct PCR reaction.

Specifications

50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns

Kit components

Part I

Buffer MP

Foregene Protease Plus

6× DNA Loading Buffer

Part II

2× M1 PCR EasyTM Mix(UNG)

Instructions

Features&advantages

■ No time-consuming and expensive DNA purification is required.

■ The sample demand is small, as little as 5mg mouse tissue or 2-5mm mouse tail can be tested.

■ No special treatments such as grinding and crushing are required, and the operation is simple.

■ The optimized PCR system makes PCR have higher specificity and stronger tolerance to PCR reaction inhibitors.

■ Anti-pollution PCR system 2×M1-PCR EasyTM Mix (UNG), which effectively eliminates the pollution caused by PCR products and ensures the specificity and accuracy of amplification.

Kit parameters

Scope of application: mouse tail, mouse ear, muscle and other tissues.

DNA released from sample lysis: only used as a PCR template.

The kit can be used for the following purposes: identification of transgenosis, animal genotyping, etc.

Work flow

wk flw-mouse tail direct PCR

Storage and Shelf life

Part I of this kit should be stored at 2-8℃.

The reagent Buffer AL can be stored for 12 months under dry conditions; it can be stored at 2-8℃ for longer storage.

Reagent Foregene Protease has a unique formula. To ensure its activity and stability, please store it at 4℃ for 12 months.

Reagent 6×DNA Loading Buffer can be stored at 4℃ or -20℃ for a long time.

Part II of this kit should be stored at -20℃.

Reagent 2×M1 PCR EasyTM Mix(UNG), if is used frequently, it can also be stored at 4℃ for short-term storage (use up within 10 days).


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