SARS-CoV-2 Variant Nucleic Acid Detection Kit II (Multiplex PCR Fluorescent Probe Method)-for detection of variants from UK,South Africa, Brazil, and India

SARS-CoV-2 Variant Nucleic Acid Detection Kit II (Multiplex PCR Fluorescent Probe Method)-for detection of variants from UK,South Africa, Brazil, and India

Kit Description:

This kit uses Real-time RT PCR technology (rRT-PCR) for qualitative detection of SARS-CoV-2 and variant lineages nucleic acids in human nasopharyngeal or oropharyngeal swab samples such as SARS-CoV-2 B.1.1.7 lineage (UK), B.1.351 lineage (ZA), B.1.617 lineage (IND) and P.1 lineage (BR).

This kit is a combo of nucleic acid purification kit and RT-qPCR detection kit for SARS-CoV-2 Variant nucleic acid. Thus there’s no need for nucleic acid purification, is economical. can complete test in 1 hour, especially suitable for large-scale rapid detection.

20+ years R&D Manufacturing Experience         25+ highest impact factor                       

1000+ different lab reagents                                100+ universities partner                         

400+ distributors                                                   2000+ SCI citations of the lab reagents 


Product Detail

Product Tags

Specifications

48 Rxns/kit, 96 Rxns/kit

No.

Component

Amount

Main ingredient

48 Rxns

96 Rxns

1

Nucleic acid release agent

1.4 mL/ tube

2 tubes

5.3 mL/bottle

1 bottle

Surfactant

2

RNA protectant

53 μL/tube

1 tube

106 μL/tube

1 tube

RNase inhibitor

3

SARS-CoV-2 Variant

reaction solution A

800 μL/tube

1 tube

1600 μL/tube

1 tube

Primer, probe, reaction buffer, dNTP

4

SARS-CoV-2 Variant

reaction solution B

800 μL/tube

1 tube

1600 μL/tube

1 tube

Primer, probe, reaction buffer, dNTP

5

SARS-CoV-2 enzyme mixture

160 μL/tube

1 tube

320 μL/tube

1 tube

Hot start Taq enzyme, M-MLV enzyme

6

SARS-CoV-2

positive control

100 μL/tube

1 tube

100 μL/tube

1 tube

Recombinant plasmid containing target fragment, RNA

7

SARS-CoV-2 negative control

1200 μL/tube

1 tube

1200 μL/tube

1 tube

TE buffer

Note:Components from different kits cannot be used interchangeably.

Features&advantages:

Quick and easy:complete the test in about 1 hour

Avoid nucleic acid loss:no need for nucleic acid purification

Low detection limit and high sensitivity, especially suitabe for large-scale rapid detection

Low equipment requirement and highly flexible use

Detection sensitivity can reach the lowest level as 500 copies/mL).

How to use:

1.Applicable instrument

ABI7500,Bio-Rad CFX96,Roche LightCycler 480,SLAN-96S

2.Detection procedure

Sample processing:Naopharyngeal or oropharyngeal swab sample (only 1 step operation)(Wait for 10 mins)

PCR amplificaton(<=55min)

Workflow:

SARS-CoV-2 SARS-CoV-3 SARS-CoV-4

Storage:

Sealed from light and stored at -20±5℃;

Store at 2-8℃ after opening, use within 7 days; avoid repeated freeze-thaw(<5 times)

Shelf life:1 year


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